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Effect of granulosa and cumulus cells on in vitro development of the bovine follicular oocytes |
K. S. Im, H. J. Kim, K. M. Chung, H. S. Kim, K. W. Park, K. Niwa |
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Abstract |
The effect of co-culture with cumulus cells and granulose cells during maturation and development on in vitro developmental potency of follicular oocytes was examined. TCM-199 supplemented with 15% FCS and hormones was used as maturation medium. Sperm from frozen semen was capaciated in modified mTALP medium containing 0.3% BSA, 10 關g/ml heparin and 5 mM/ml caffeine. The fertilized embryos were co-cultured on monolayer of cumulus cells or granulose cells in TCM-199. The embryo co-cultured with cumulus cells showed higher percentage of embryo developed to morula and blastocyst(73.3%) than the embryo co-cultured without cumulus cells(30.8%). The percentage of oocytes developed to morula and blastocyst among cleaved oocytes was significantly(p<0.05) higher in the oocytes co-cultured with cululus cells during development(62.4%) than in the oocytes co-cultured with granulose cells during maturation and with cumulus cells during development(52.3%), and in the oocytes co-cultured with granulose cells during development(52.8%). The results of this study indicate that co-culture of in vitro fertilized embryos with cumulus cells in the development medium increased the rate of embryos developed to morula and blastocyst among cleaved oocytes. |
Keywords:
Follicular Oocyte; Co-culture; Cumulus Cells; Granulosa Cells; Blastocyst |
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