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Influence of phenyulalanine in the medium on protein synthesis of chicken embryo fibroblasts |
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K. Kita, M. Miyazaki, J. Okumura |
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| Abstract |
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The influence of phenylalanine(Phe) in the medium on protein synthesis of chicken embryo fibroblasts(CEF) was examined. CEF was derived from 9-d-old embryos by trypsin-EDTA digestion. To examine the deficiency of Phe in the medium, CEF was cultured in Dulbecco`s modified Eagle`s medium(DMEM) with or without Phe. CEF was also cultured in Dulbecco`s phosphate buffered saline(PBS(Ca2+, Mg2+)) with or without 400 關M Phe in order to examine the effect of Phe supplementation. All media were supplemented with 10% (v/v) fetal calf serum. After incubation for 6, 30 and 54 h, protein synthesis was measured by the incorporation of L-[2, 6-3H] Phe into CEF for further 18 h. Protein synthesis of CEF cultured in DMEM was higher than that in PBS(Ca2+, MG2+). High specific radioactivity of Phe due to the low concentration of Phe in the medium resulted in the apparent increase in protein synthesis of CEF. Protein synthesis cultured in PBS(Ca2+, Mg2+) with Phe did not increase during 72 h of cell culture. |
| Keywords:
Protein Synthesis; Phenylalanine; Chicken Embryo Fibroblast |
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