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Assessment of Bandsharing Values in RAPD-PCR Analysis of Dwarf Cattle of Kerala |
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P. Suprabha, K. Anilkumar, T. V. Aravindakshan, K. V. Raghunandanan |
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| Abstract |
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Random amplified polymorphic DNA (RAPD-PCR) analysis of 56 animals of four different genetic groups of dwarf cattle in Kerala was done as a single step analysis. Bandsharing (BS) values were calculated for animals of each group and between groups as an analytical tool to find out genetic variation among animals. The different factors affecting BS values were estimated using Harvey`s Least squares analysis. The effects of genetic group, Guanine-cytosine (GC) content of primer and gel on BS values were found significant. Bandsharing values of Kasargode-Highrange dwarf animals were significantly different from Vechur, Vatakara and their combinations. The Vechur, Vatakara and Vechur-Vatakara combinations were found to be more uniform (high BS value) compared with other combinations. The bandsharing value was lowest with primers of GC content 90% and highest with 80% GC content. The effect of gel on BS value points to the need of adjustments of gel factor for calculation of BS values. |
| Keywords:
Random Amplified Polymorphic DNA Marker; Bandsharing Value; Dwarf Cattle |
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