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Retroviral Gene Expression in Spermatogonial Stem Cells during Long-term Culture |
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D. K. Jeong*, Michael D. Griswold |
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Correspondence:
D. K. Jeong,
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| Abstract |
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The spermatogonial stem cell (SSCs) is unique in that it is the only cell in the adult male that can contribute genes to a subsequent generation. Permanent modification of the germ cell line may be realized if stem cells could be cultured, transfected with unique genes, and then transplanted into recipient testes. We developed a culture system that supported long-term viability of SSCs. We used a retrovirus vector (pMSCV including -galactosidase) to stably transfect spermatogonia following long-term culture using the system developed. Expression of the reporter gene -galactosidase controlled by the retroviral vector was stable in long-term cultured SSCs. We confirmed the retroviral-mediated -galactsidase gene could be expressed in germ cells in recipient mice following SSCs transplantation. |
| Keywords:
Retrovirus; Gene Transfer; Electroporation; Stem Cell Medium |
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