Supplementation Effects of C18:2 or C18:3 Rich-oils on Formations of CLA and TVA, and Lipogenesis in Adipose Tissues of Sheep

Choi, S. H.; Lim, K. W.; Lee, H. G.; Kim, Y. J.; Song, M. K.

doi:2007.20.9.1417

Article

Ruminant Nutrition and Forage Utilization

Asian-Australasian Journal of Animal Sciences 2007;20(9): 1417-1423.
https://doi.org/10.5713/ajas.2007.1417 Published online August 30, 2007.

Supplementation Effects of C18:2 or C18:3 Rich-oils on Formations of CLA and TVA, and Lipogenesis in Adipose Tissues of Sheep

S. H. Choi, K. W. Lim, H. G. Lee, Y. J. Kim, M. K. Song

Abstract

The present study was conducted to investigate the supplementation effects of C18:2 rich-soybean oil or C18:3 rich-perilla oil (7% of total diet, DM basis) for 12 weeks on plasma metabolites, fatty acid profile, in vitro lipogenesis, and activities of LPL and FAS in adipose tissue of sheep. The treatments were basal diet (Control), C18:2 rich-soybean oil supplemented diet (SO-D) and C18:3 rich-perilla oil supplemented diet (PO-D). All the sheep were fed the diets consisting of roughage to concentrate in the ratio of 40:60 (DM basis). Oil supplemented diets (SO-D and PO-D) slightly increased contents of triglyceride (TG) and total cholesterol (TC), proportions of both cis-9 trans-11 and trans-10 cis-12 CLA and TVA, but lowered (p<0.01) those of C18:0 compared to the control diet. No differences were observed in the contents of TG and TC and proportions of fatty acids in plasma between supplemented oils. Oil supplemented diets slightly increased the proportions of cis-9 trans-11 and trans-10 cis-12 types of CLA in subcutaneous adipose tissue of sheep compared to the control diet. The rate of lipogenesis with acetate was higher (p<0.01) for intermuscular- and subcutaneous adipose tissues than that for intramuscular adipose tissue, while that with glucose did not differ among fat locations in sheep fed SO-D. No differences were observed in the rate of lipogenesis between substrates in all fat locations. The rates of lipogenesis with glucose increased only in the intermuscular- (p<0.01) and subcutaneous adipose tissue (p<0.005) compared to those with acetate. The rates of lipogenesis with acetate were the highest in the intermuscular and intramuscular adipose tissue of the sheep fed PO-D. Oil supplemented diets slightly increased the rate of lipogenesis with glucose for all fat locations. Supplementation of oils to the diet numerically increased the fatty acid synthase activity but did not affect the lipoprotein lipase activity in subcutaneous adipose tissue.

Keywords: Conjugated Linoleic Acid; Oils; Lipogenesis; Lipogenic Enzymes; Adipose Tissue; Sheep