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An Antisense Oligodeoxynucleotide to the LH Receptor Attenuates FSH-induced Oocyte Maturation in Mice |
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Jiange Yang, Maoyong Fu, Songbo Wang, Xiufen Chen, Gang Ning, Baoshan Xu, Yuzhen Ma, Meijia Zhang, Guoliang Xia* |
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Correspondence:
Guoliang Xia,
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| Abstract |
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It has been recently shown that expression of the LH receptor (LHR) in cumulus cells is related with FSH-induced meiotic resumption of mouse cumulus enclosed oocytes (CEOs). However, to date, it is still unclear whether LHR expression in cumulus cells plays a key role during FSH-induced oocyte maturation. The purpose of this study was to characterize the functional role of LHRs in cumulus cells. CEOs were isolated from eCG-primed preovulatory follicles and cultured in hypoxanthine (HX) arrested medium. LHR protein expression in cumulus cells was time-dependent increasing during the process of FSH-induced oocyte maturation. While the sense oligodeoxynucleotide (ODN) had no effect, antisense ODN inhibited FSH-induced LHR expression and meiotic resumption. Moreover, this antisense ODN against LHR could inhibit FSH-induced mitogen-activated protein kinase (MAPK) phosphorylation. This study suggested that LHR expression in cumulus cells is involved in FSH-induced oocyte meiotic resumption, which process is possibly regulated by MAPK cascade. |
| Keywords:
Cumulus-enclosed Oocyte; LH Receptor; Oligodeoxynucleotide; Mouse |
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