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Asian-Australas J Anim Sci > Accepted
doi: https://doi.org/10.5713/ajas.16.0773    [Accepted]
Effects of sodium diacetate on the fermentation profile, chemical composition and aerobic stability of alfalfa silage
XianJun Yuan1, AiYou Wen1,2, Seare T. Desta1, Jian WANG1, Tao Shao1,*
1Institute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing, China
2College of Animal Science,Anhui Science and Technology University,Fengyang, 233100,China
* Corresponding Author: Tao Shao ,Tel: +86 025 84396130, Fax: +86 025 84396130, Email: shaotaola@163.com
Received: 9 October 2016;  Revised: 16 November 2016.  Accepted: 17 December 2016.  Published online 26 December 2016.

ABSTRACT
Objective:
The objective of this study was to evaluate the effect of sodium diacetate (SDA) on fermentation profile, chemical composition and aerobic stability of alfalfa (Medicago sativa L.) silage.
Method:
Fresh alfalfa was ensiled with various concentrations of SDA (0, 3, 5, 7 and 9 g/kg of fresh forage). After 60 days of the ensiling, the samples were collected to examine the fermentative quality, chemical composition and aerobic stability.
Results:
The application of SDA significantly (P<0.05) decreased silage pH with the lowest value in silage with 7 g/kg of SDA. The proliferations of enterobacteria, yeasts, molds and clostridia were inhibited by SDA, resulted in lower ethanol, propionic and butyric acid concentrations and dry matter loss in SDA treated silages than control. The increasing SDA linearly decreased free amino acid N (P<0.001), ammonia N (P=0.018) and non-protein N (P<0.001), while linearly increased water soluble carbohydrate (P<0.001) and peptide N (P<0.001). It is speculated that SDA accelerated the shift from homofermentative to heterofermentative lactic acid bacteria during the silage fermentation, indicated by lower lactic acid production in SDA-9 than SDA-7 silages after 60 days of ensiling. Alfalfa silages treated with SDA at 7 g/kg had highest Flieg’s point and retained stable more than 9 d during aerobic exposure under humid and hot conditions in southern China.
Conclusion:
Sodium diacetate may be used as an additive for alfalfa silages at a level of 7 g/kg.
Keywords: alfalfa; fermentation quality ; sodium diacetate; silage
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