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Asian-Australas J Anim Sci > Accepted
doi: https://doi.org/10.5713/ajas.16.0776    [Accepted]
Exercise induced upregulation of Glutamate-cysteine ligase, catalytic subunit (GCLC) and Glutamate-cysteine ligase, modifier Subunit (GCLM) gene expression in Thoroughbred horses
Jeong-Woong Park, Jae-Young Choi, Seul A Hong, Nam Young Kim3, Kyoung-Tag Do2, Ki-Duk Song1,* , Byung-Wook Cho*
Department of Animal Science, College of Natural Resources and Life SciencesPusan National University, Miryang, Korea
1Department of Animal Biotechnology, Chonbuk National University, Jeonju, Korea
2Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju, Korea
3National Institute of Animal Science, Rural Development Administration , Jeju, Korea
* Corresponding Author: Ki-Duk Song ,Tel: 010-5622-1158, Fax: 82263704848, Email: kiduk.song@gmail.com / Byung-Wook Cho ,Tel: 010-5622-1158, Fax: 82263704848, Email: kiduk.song@gmail.com
Received: October 8, 2016;  Revised: November 6, 2016.  Accepted: October 8, 2016.  Published online January 2, 2017.

ABSTRACT
Athletic ability is an important economic trait in horses, and molecular biological research on horses’ athletic abilities can lead to improvements in the animals’ economic value. In a previous study, we conducted RNA sequencing analyses of the blood and muscles of horses during exercise and identified differentially expressed genes (DEGs) in response to exercise. Among them, glutamate-cysteine ligase catalytic subunit (GCLC) and glutamate-cysteine ligase modifier subunit (GCLM) genes, of which products form glutamate cysteine ligase. In the present study, we performed molecular and gene-expression analyses of these genes in Thoroughbred horses to gain insights into their role in the oxidative stress responses that are induced during exercise. Expression of GCLC showed the same pattern in both blood and muscle tissues after exercise. Expression of GCLC increased in the muscle and blood of Thoroughbreds, suggesting a tissue-specific regulatory mechanism for the expression of GCLC. In addition, expression of the GCLM gene increased after exercise in both the blood and muscle of Thoroughbreds. These results show that expression of GCLC and GCLM, which are regulators of oxidative stress and reactive oxygen species (ROS), may be regulated to cope with the oxidative stress caused by exercise. Thus, we established the expression patterns of GCLC and GCLM in the skeletal muscle and blood of Thoroughbred horses in response to exercise. Further study is now warranted to uncover the functional importance of these genes in exercise and recovery in racehorses.
Keywords: Horse; exercise; GCLC (glutamate-cysteine ligase catalytic subunit; GCLM (glutamate-cysteine ligase modifier subunit); Quantitative Polymerase Chain Reaction
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