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Asian-Australas J Anim Sci > Accepted
doi: https://doi.org/10.5713/ajas.16.0677    [Accepted]
Physico-chemical properties of late-incubation egg amniotic fluid and a potential in ovo feed supplement
Apeh A Omede1,2 , Momenuzzaman M Bhuiyan1, Fakrul A Islam1, Paul A Iji1,*
1School of Environmental & Rural Science, University of New England, Armidale NSW 2351, Australia
2Department of Animal Production, Kogi State University, PMB 1008, Anyigba, Nigeria
* Corresponding Author: Paul A Iji ,Tel: 61267732082, Fax: 61267733922, Email: piji@une.edu.au
Received: September 7, 2016;  Revised: October 7, 2016.  Accepted: January 25, 2017.  Published online January 26, 2017.

ABSTRACT
This study explored the physico-chemical properties of late-incubation egg amniotic fluid and a potential in ovo feed (IOF) supplement. Amniotic fluid was collected from broiler breeders (Ross 308, 51 weeks and Cobb 500, 35 weeks) on day 17 after incubation. A mixture of high-quality soy protein supplement – Hamlet Protein AviStart (HPA) was serially diluted in MilliQ water to obtain solutions ranging from 150 to 9.375 mg/ml. The mixtures were heat-treated (0, 30, 60 minutes) in a waterbath (80 oC) and then centrifuged to obtain supernatants. The amniotic fluid and HPA supernatants were analysed for their physico-chemical properties. Only viscosity and K+ were significantly (P < 0.05) different in both strains. Of all essential amino acids, leucine and lysine were in the highest concentration in both strains. The osmolality, viscosity and pCO2 of the supernatants decreased (P < 0.05) with decreasing HPA concentration. Heat treatment significantly (P < 0.05) affected osmolality, pH, and pCO2, of the supernatants. The interactions between HPA concentration and heat treatment were significant with regards to osmolality (P < 0.01), pH (P < 0.01), pCO2 (P < 0.05), glucose (P < 0.05), lactate (P < 0.01) and acid-base status (P < 0.01) of HPA solutions. The Ca2+, K+, glucose, and lactate increased with increasing concentration of HPA solution. The protein content of HPA solutions decreased (P < 0.05) with reduced HPA solution concentrations. The supernatant from 150 mg/ml HPA solution was richest in glutamic acid, aspartic acid, arginine and lysine. Amino acids concentrations were reduced (P < 0.05) with each serial dilution but increased with longer heating. The values obtained in the primary solution (highest concentration) are close to the profiles of high-protein ingredients. This supplement, as a solution, hence, may be suitable for use as an IOF supplement and should be tested for this potential.
Keywords: Amino acids; amniotic fluids; in ovo; physico-chemical properties; protein
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