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Asian-Australas J Anim Sci > Accepted
doi: https://doi.org/10.5713/ajas.16.0744    [Accepted]
Evaluation of Microbiological, Cellular and Risk Factors Associated with Subclinical Mastitis in Female Buffaloes
Emmanuella Oliveira Moura, Adriano Henrique do Nascimento Rangel, Maria Celeste Nunes de Melo2, Luiz Henrique Fernandes Borba, Dorgival Morais de Lima Júnior1, Luciano Patto Novaes, Stela Antas Urbano*, Júlio César de Andrade Neto
Universidade Federal do Rio Grande do Norte, Natal/RN, Brazil
1Universidade Federal de Alagoas, Arapiraca, Brazil
2Universidade Federal do Rio Grande do Norte, Departamento de Microbiologia e Parasitologia, Natal/RN, Brasil, 59078-970
* Corresponding Author: Stela Antas Urbano ,Tel: +55 84 99921-4650, Fax: +55 84 3223-4583, Email: stela_antas@yahoo.com.br
Received: September 30, 2016;  Revised: November 9, 2016.  Accepted: February 1, 2017.  Published online February 1, 2017.

ABSTRACT
Objective:
This study aimed to evaluate the microbiological and cellular milk profile for the diagnosis of subclinical mastitis in female buffaloes and to assess risk factors for predisposition of the disease.
Method:
Analyses were carried out by standard plate count (SPC), identification of species and antibiotic resistance, somatic cell count (SCC), electrical conductivity of milk (ECM), and Lactoferrin content in milk (Lfe). Teat cups were swabbed to evaluate risk factors, observing hyperkeratosis, milking vacuum pressure and cleanliness of the site. Hence, 30 female buffaloes were randomly selected (15 from a group in early lactation and 15 in late lactation).
Results:
The most common bacteria in the microbiological examination were Staphylococcus spp., Streptococcus spp. and Corynebacterium sp. In the antibiotic sensitivity test, 10 (58.82%) of the 17 antibiotics tested were sensitive to all isolates, and resistant bacteria were S. uberis, S. dysgalactiae, S. haemolyticus and E. coli. It was observed that positive samples in the microbiological examination showed total bacterial count (TBC) between 9.10 x 103 to 6.94 x 106 CFU/mL, SCC between 42,000 to 4,320,000 cells/mL and ECM ranging from 1.85 to 7.40 mS/cm. It was also found that the teat cups had high microbial counts indicating poor hygiene, and even faults in the cleanliness of the animals’ waiting room were observed. It is concluded that values of SCC above 537,000 cells/mL and ECM above 3.0 mS/mL are indications of mammary gland infection for this herd; however, the association of these values with a microbiological analysis is necessary to more accurately evaluate the health status of mammary glands with subclinical mastitis.
Conclusion:
Through phenotypic characterization of bacteria involved in the samples, the genera Staphylococcus spp., Streptococcus spp. and Corynebacterimum bovis were the most prevalent in this study. Faults in environment and equipment hygienization are factors that are directly associated with mastitis.
Keywords: Bubaline; SCC; Quality; Production
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