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Asian-Australas J Anim Sci > Accepted
doi: https://doi.org/10.5713/ajas.16.0376    [Accepted]
Coat Colour Phenotype of Qingyu Pig Is Associated With Polymorphisms of Melanocortin Receptor 1 Gene
Xiaoqian Wu, Zhendong Tan, Linyuan Shen, Qiong Yang1, Xiao Cheng, Kun Liao2, Lin Bai, Surong Shuai, Mingzhou Li, Xuewei Li, Shunhua Zhang*, Li Zhu*
Sichuan Agricultural University, Chengdu, China
1Chengdu Agricultural College, Chengdu, China
2Pasturage Station of Tongjiang Agriculture Bbureau, Bazhong, China
* Corresponding Author: Shunhua Zhang ,Tel: 86 2886291010, Fax: 86 2886291010, Email: zhuli7508@163.com / Li Zhu ,Tel: 86 2886291010, Fax: 86 2886291010, Email: zhuli7508@163.com
Received: 11 May 2016;  Revised: 10 August 2016.  Accepted: 10 December 2016.  Published online 17 December 2016.

ABSTRACT
Melanocortin receptor 1 (MC1R) and Agouti signaling protein (ASIP) are two widely reported pivotal genes that significantly affect the regulation of coat colour. Qingyu pig, a Chinese indigenous pig breed, exhibits two types of coat colour phenotypes, including pure black and white with black spotting respectively. The objectives of this study were to investigate whether the polymorphisms of these two genes are associated with coat colour and analyze the molecular mechanism of the coat colour separation in Qingyu pig. We studied the phenotype segregation and investigated the polymorphism of MC1R and ASIP in 121 Qingyu pigs, consisting of 115 black and 6 white with black spotted pigs. Our result showed that coat colour of Qingyu pig is associated with the polymorphisms of MC1R but not ASIP. We only found 2 haplotypes, EQY and Eqy, based on the 13 observed SNPs from MC1R gene. Among which, Eqy presented a recessive inheritance mode in black spotted Qingyu pigs. Further analysis revealed a g.462-463CC insertion that caused a frameshift mutation and a premature stop codon, thus changed the first transmembrane domain completely and lost the remaining six transmembrane domains. Overall, our findings indicated that black coat colour in Qingyu pig was dominant to white with black spotted phenotype and MC1R gene polymorphism was associated with coat colour separation in Qingyu pig.
Keywords: Qingyu pig; Coat colour; MC1R; ASIP; Polymorphism
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