Department of Chemical, Biological & Environmental Engineering, Hanjiang Normal University, Hubei, Shiyan 442000, China 1Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, wuhan, Korea
Received: October 15, 2015; Revised: November 15, 2015. Accepted: December 17, 2016. Published online December 27, 2016.
Objective: The aim of this study was to investigate the effects of simultaneous supplementation of laying hen with a-linolenic acid (ALA) resources (flax, perilla and Eucommia ulmoides seeds) and eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA) resources (Schizochytrium sp.) on egg quality and n-3 polyunsaturated fatty acids (PUFA) profile.
Method: Dietary treatments were as follows: i) diet C (control diet); ii) diet F( diet C+ 10% flaxseeds ); ⅲ) diet P, (diet C + 10% perilla seeds); ⅳ) diet E (diet C+ 10% Eucommia ulmoides seeds); ⅴ) diet A (diet C + 1.5% microalage); ⅵ) diet AF ( diet C + 10% flaxseeds + 1.5% microalage); ⅶ) diet AP (diet C + 10% perilla seeds + 1.5% microalage ) ; ⅷ) diet AE (diet C+10% Eucommia ulmoides seeds + 1.5% microalage ).
Results: Egg weight, yolk weight and production ratio were not significantly affected by either algae or in combination with seeds (P>0.05). No significant difference was observed in ALA and DHA concentration in eggs between flaxseed, perilla and eucommia ulmodies seeds supplementation alone (P>0.05). N-3 PUFA in eggs was slightly improved by microalgae supplementation. The best supplementation, a combination of microalgae and perilla seeds, elevated (P<0.05) ALA from 19.7 to 202.5 mg/egg and EPA + DHA from 27.5 to 159.7 mg/egg. Highest n-3 PUFA enrichment (379.6 mg/yolk) was observed with supplementation of a combination of perilla seed and microalgae (362.2 mg/yolk), followed by a combination of flaxseed and microalgae (348.4 mg/yolk). The ALA, EPA and DHA content obtained with a combination of microalgae and seeds overpassed the total sum of that with microalgae or ALA-seeds alone .
Conclusion: It is feasible to enrich eggs with n-3 PUFAs by perilla or eucommia ulmodies seeds instead of flaxseeds. Simultaneous supplementation of microalgae and seeds helped improve the transfer from EPA and DPA into DHA.
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