1Dept. of Biotechnology, Daegu University, Gyeongbuk, Korea 2Futuristic Animal Resource and Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk, Korea 3College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University, Chuncheon, Korea 4College of Pharmacy, Kangwon National University, Chuncheon, Korea 5Dept. of Animal Science, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea 6National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk, Korea 7Maria Fertility Hospital, Daegu, Korea
Received: December 23, 2016; Revised: February 4, 2017. Accepted: February 19, 2017. Published online February 23, 2017.
Objective: Phellodendron amurense (P. amurense) and Humulus japonicus (H. japonicus) are closely involved in anti-oxidative response and increasing antioxidant enzymes activities. However, the effects of their extracts on development of preimplantation bovine embryos have not yet been investigated. Therefore, we investigated the effects of P. amurense and H. japonicus extract on developmental competence and quality of preimplantation bovine embryos.
Method: After in vitro fertilization, bovine embryos were cultured for 7 days in CR1aa medium supplemented with P. amurense (0.01 μg/ml) and H. japonicus (0.01 μg/ml). The effect of P. amurense and H. japonicas during in vitro culture on development competence and antioxidant was investigated.
Results: We observed that the blastocysts rate was significantly increased (p < 0.05) in P. amurense (28.9 ± 2.9%), H. japonicus (30.9 ± 1.5%), and a mixture of P. amurense and H. japonicus (34.8 ± 2.1%) treated groups compared with the control group (25.4 ± 1.6%). We next confirmed that the intracellular levels of reactive oxygen species (ROS) were significantly decreased (p < 0.01) in P. amurense and/or H. japonicus extract treated groups when compared with the control group. Our results also showed that expression of cleaved caspase-3 and apoptotic cells of blastocysts were significantly decreased (p < 0.05) in bovine blastocysts derived from both P. amurense and H. japonicus extract treated embryos.
Conclusion: These results suggest that proper treatment of P. amurense and H. japonicus extracts in the development of preimplantation bovine embryos improves the quality of blastocysts, which may be related to the reduction of ROS level and apoptosis.
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