Anim Biosci > Volume 33(7); 2020 > Article |
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Item | Starter phase1) (1 to 21 d) | Grower-finisher phase1) (22 to 42 d) | ||||
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Control | 0.4% COAC | 0.8% COAC | Control | 0.4% COAC | 0.8% COAC | |
Ingredients | ||||||
Corn | 60.00 | 60.00 | 60.00 | 66.00 | 66.00 | 66.00 |
Soybean meal | 28.50 | 28.50 | 28.50 | 24.00 | 24.00 | 24.00 |
Fish meal | 2.00 | 2.00 | 2.00 | 1.00 | 1.00 | 1.00 |
Wheat middlings | 4.50 | 4.50 | 4.50 | 4.00 | 4.00 | 4.00 |
Salt | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 |
Choline chloride (50%) | 0.15 | 0.15 | 0.15 | 0.10 | 0.10 | 0.10 |
Calcium hydrophosphate | 1.20 | 1.20 | 1.20 | 1.00 | 1.00 | 1.00 |
Limestone | 1.20 | 0.90 | 0.60 | 1.20 | 0.90 | 0.60 |
Compound organic acid calcium | 0.00 | 0.40 | 0.80 | 0.00 | 0.40 | 0.80 |
Zeolite power | 1.15 | 1.05 | 0.95 | 1.40 | 1.30 | 1.20 |
Premix2) | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
Total | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 |
Nutrient levels3) | ||||||
ME (MJ/kg) | 11.73 | 11.73 | 11.73 | 11.91 | 11.91 | 11.91 |
Crude protein | 18.86 | 18.86 | 18.86 | 16.69 | 16.69 | 16.69 |
Lysine | 1.21 | 1.21 | 1.21 | 1.08 | 1.08 | 1.08 |
Methionine | 0.57 | 0.57 | 0.57 | 0.45 | 0.45 | 0.45 |
Calcium | 0.98 | 1.00 | 0.98 | 0.87 | 0.87 | 0.86 |
Total phosphorus | 0.70 | 0.70 | 0.70 | 0.61 | 0.61 | 0.61 |
Aailable phosphorus | 0.46 | 0.46 | 0.46 | 0.39 | 0.39 | 0.39 |
1) Control = basal diet without any feed additive; 0.4% COAC = basal diet + 0.4% compound organic acid calcium; 0.8% COAC = basal diet + 0.8% compound organic acid calcium.
2) Premix supplied the following per kilogram of diet: vitamin A 25,000 IU; vitamin D 5,000 IU; vitamin E (DL-α-tocopheryl acetate), 12.5 IU; vitamin K (menadione) 1.25 mg; vitamin B1 (thiamine) 1.0 mg; vitamin B2 (riboflavin) 8 mg; vitamin B6 (pyridoxine) 3.0 mg; vitamin B12 (cyanocobalamin) 15 μg; folic acid 250 μg; nicotinic acid 17.5 mg; calcium pantothenate 12.5 mg; Fe 80 mg; Cu 10 mg; Mn 80 mg; Zn 80 mg; Se 0.15 mg; I 0.35 mg; phytase 500 U.
Items | 21 days of age1) | SEM | p-value | ||
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Control | 0.4% COAC | 0.8% COAC | |||
Body weight (g) | |||||
1 day of age | 45.80 | 45.19 | 45.40 | 0.35 | 0.52 |
21 days of age | 563.45a | 600.15b | 594.71b | 9.91 | 0.04 |
42 days of age | 1,498.89a | 1,608.86b | 1,603.45b | 35.31 | 0.01 |
1 to 21 days of age | |||||
ADFI (g) | 42.68 | 44.02 | 43.43 | 0.74 | 0.50 |
ADG (g) | 24.65a | 26.43b | 26.16b | 0.48 | 0.04 |
F/G | 1.73b | 1.67a | 1.66b | 0.01 | 0.01 |
22 to 42 days of age | |||||
ADFI (g) | 93.43 | 95.59 | 95.98 | 1.62 | 0.55 |
ADG (g) | 44.54a | 48.03b | 48.04b | 1.51 | 0.02 |
F/G | 2.10b | 1.99a | 2.00a | 0.04 | 0.01 |
1 to 42 days of age | |||||
ADFI (g) | 67.99 | 69.77 | 69.71 | 0.95 | 0.37 |
ADG (g) | 34.60a | 37.23b | 37.10b | 0.85 | 0.01 |
F/G | 1.97b | 1.87a | 1.88a | 0.03 | 0.02 |
COAC, compound organic acid calcium; SEM, standard error of means for 6 broilers each; ADFI, the average daily feed intake; ADG, the average daily gain; F/G, the ratio of feed gain.
Items | 21 days of age1) | SEM | p-value | 42 days of age1) | SEM | p-value | ||||
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Control | 0.4% COAC | 0.8% COAC | Control | 0.4% COAC | 0.8% COAC | |||||
Biochemical constituents | ||||||||||
GOT (U/L) | 29.87 | 30.48 | 35.28 | 2.648 | 0.302 | 20.55 | 19.95 | 20.5 | 0.833 | 0.867 |
GPT (U/L) | 2.2 | 1.54 | 1.26 | 0.35 | 0.141 | 2.43 | 1.70 | 2.46 | 0.473 | 0.461 |
AKP (U/L) | 17.07 | 19.63 | 17.06 | 2.106 | 0.672 | 87.32b | 65.93a | 94.29b | 8.007 | 0.022 |
TP (g/L) | 11.12 | 11.5 | 11.69 | 1.27 | 0.700 | 10.81 | 10.86 | 19.96 | 1.260 | 0.970 |
Antioxidants enzymes | ||||||||||
T-AOC (U/mL) | 1.39 | 1.53 | 1.46 | 0.062 | 0.282 | 2.34 | 2.96 | 2.53 | 0.419 | 0.626 |
T-SOD (U/mL) | 295.09 | 324.75 | 323.76 | 20.788 | 0.584 | 95.06 | 99.43 | 104.08 | 7.534 | 0.755 |
GSH-Px (U/L) | 23.02 | 23.06 | 23.15 | 1.453 | 0.998 | 25.97 | 24.03 | 29.9 | 3.194 | 0.476 |
MDA (nmol/mL) | 0.57 | 0.38 | 0.45 | 0.071 | 0.143 | 2.02 | 1.62 | 1.31 | 0.236 | 0.093 |
COAC, compound organic acid calcium; SEM, standard error of means for 6 broilers each; GOT, glutamic oxalacetic transaminase; GPT, aspartate amino transferase; AKP, alkaline phosphatase; TP, total protein; T-AOC, total antioxidant capacity; T-SOD, total superoxide dismutase; GSH-Px, glutathione peroxidase; MDA, malondialdehyde.