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Effect of Codonopsis pilosula polysaccharide on the quality of sheep semen preservation at 4°C |
Yuqin Wang1 
, Yanhong Zhao2
, Huan Chen3,4
, Tingting Lu1
, Rujie Yang2
, Xiuxiu Weng1
, Wanhong Li1,*
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1National Demonstration Center for Experimental Grassland Science Education, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Gansu Lanzhou 730020, China
2Tianzhu Animal Breeding Research Institute, Gansu Wuwei 733000, China
3Wuwei Animal Husbandry and Veterinary Science Research Institute, Gansu Wuwei 733000, China
4Gansu Lantian Tonghe Co. Ltd., Gansu Wuwei 733000, China |
Correspondence:
Wanhong Li, Tel: +86-0931-891-4266, Fax: +86-0931-891-4266, Email: limh@lzu.edu.cn
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Received: 13 July 2023 • Revised: 17 September 2023 • Accepted: 27 November 2023 |
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| Abstract |
Objective
This study aimed to investigate the effect of Codonopsis pilosula polysaccharide (CPP) on the motility, mitochondrial integrity, acrosome integrity rate, and antioxidant ability of sheep sperm after preservation at 4°C.
Methods
Semen from healthy adult rams were collected and divided into four groups with separate addition of 0, 200, 400, and 1,000 mg/L CPP. Sperm motility was analyzed using the Computer-Assisted Semen Analysis software after preservation at 4°C for 24, 72, 120, and 168 h. Sperm acrosome integrity rate was analyzed by Giemsa staining at 24, 72, and 120 h, and mitochondrial membrane integrity was analyzed by Mito-Tracker Red CMXRos. The total antioxidant capacity (T-AOC) and malondialdehyde (MDA) content of spermatozoa were measured after 120 h of preservation.
Results
The sperm viability and forward-moving sperm under 200 mg/L CPP were significantly higher than that in the control group at 72 h (61.28%±3.89% vs 52.83%± 0.70%, 51.53%±4.06% vs 42.84%±1.14%), and 168 h (47.21%±0.85% vs 41.43%±0.37%, 38.68%±0.87% vs 31.68%±0.89%). The percentage of fast-moving sperm (15.03%±1.10% vs 11.39%±1.03%) and slow-moving sperm (23.63%±0.76% vs 20.29%±1.11%) in the 200 mg/L group was significantly higher than control group at 168 h. The mitochondrial membrane integrity of the sperm in the group with 200 mg/L CPP was significantly higher than those in the control group after storage at 4°C for 120 h (74.76%±2.54% vs 65.67% ±4.51%, p<0.05). The acrosome integrity rate in the group with 200 mg/L (87.66%±1.26%) and 400 mg/L (84.00%±2.95%) was significantly higher than those in the control group (80.65%±0.16%) after storage for 24 h (p<0.05). CPP also increased T-AOC and decreased the MDA concentration after preservation at 4°C (p<0.05).
Conclusion
Adding CPP could improve the T-AOC of sperm, inhibit lipid peroxidation, and facilitate semen preservation. |
| Keywords:
Codonopsis pilosula Polysaccharide; Motility; Sheep; Sperm |
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